부산대학교 도서관

We have developed a versatile, potent technique for imaging cells in culture and in vivo by expressing a metabolically biotinylated cell-surface receptor and visualizing it with labeled streptavidin moieties. The recombinant reporter protein, which incorporates a biotin acceptor peptide (BAP) between an N-terminal signal sequence and a transmembrane domain, (BAP-TM) was efficiently biotinylated by endogenous biotin ligase in mammalian cells with the biotin displayed on the cell surface. Tumors expressing the BAP-TM have high sensitivity for magnetic resonance and fluorescence tomographic imaging in vivo after intravascular injection of streptavidin conjugated to magnetic nanoparticles or fluorochromes, respectively. Moreover, streptavidin-horseradish peroxidase conjugates in conjunction with a peroxidase-sensitive gadolinium agent further increased and prolonged the magnetic resonance signal. This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo. *Note: In the version of this article originally published, reference 12 was incorrect. The correct reference 12 is Querol, M., Chen, J.W., Weissleder, R. Bogdanov, A. Jr. DTPA-bis-amide based MR sensor agents for peroxidase imaging. Org. Lett. 17, 1719-1722 (2005). This error has been corrected in the PDF version of the article.
Author(s): Bakhos A Tannous [1, 2, 3, 4]; Jan Grimm [1, 4]; Katherine F Perry [2]; John W Chen [1]; Ralph Weissleder (corresponding author) [1]; Xandra O Breakefield [1, 2, [...]