학술논문
Regulatory Effects of Stevia Rebaudiana on NADPH Oxidase-Related Manifestations of Oxidative Stress in Diabetic Rats with Spinal Cord Injury
Document Type
Academic Journal
Author
Source
Neurophysiology. January 2021, Vol. 53 Issue 1, p13, 9 p.
Subject
Language
English
ISSN
0090-2977
Abstract
INTRODUCTION As is known, oxidative and nitrosative stresses play an important role in the progression of spinal cord injury (SCI). Concurrent upregulations of NADPH oxidase (Nox), eNOS, and iNOS work [...]
For the first time, a method for isolation and purification of Nox isoforms (Nox1+Nox2), as well as of [O.sub.2.sup.-]-producing stable associates of Nox and lipoprotein-containing NADPH isoforms (NCL associates), from the membranes of cells and intracellular formations of the spinal cord (SC) and bone marrow (BM) of albino rats was developed, and the respective estimates were obtained. The effects of treatment with a phytopreparation of Stevia (Stevia rebaudiana Bertoni) leaves in this pathology were studied in a model of fructose-induced type II diabetes (FID) in rats with spinal cord injury (SCI). Due to its antioxidant activity, Stevia exhibited a clear membrane-stabilizing effect in rats with FID and SC hemisection. In such experimental animals, the qualitative and quantitative indices for Nox isoforms and NCL associates from the SC and BM membranes showed clear trends toward normalization. Keywords: diabetic rats, spinal cord injury, oxidative stress, Nox isoforms, NADPH-containing lipoprotein associates (NCL), Stevia phytopreparation, Suprol.
For the first time, a method for isolation and purification of Nox isoforms (Nox1+Nox2), as well as of [O.sub.2.sup.-]-producing stable associates of Nox and lipoprotein-containing NADPH isoforms (NCL associates), from the membranes of cells and intracellular formations of the spinal cord (SC) and bone marrow (BM) of albino rats was developed, and the respective estimates were obtained. The effects of treatment with a phytopreparation of Stevia (Stevia rebaudiana Bertoni) leaves in this pathology were studied in a model of fructose-induced type II diabetes (FID) in rats with spinal cord injury (SCI). Due to its antioxidant activity, Stevia exhibited a clear membrane-stabilizing effect in rats with FID and SC hemisection. In such experimental animals, the qualitative and quantitative indices for Nox isoforms and NCL associates from the SC and BM membranes showed clear trends toward normalization. Keywords: diabetic rats, spinal cord injury, oxidative stress, Nox isoforms, NADPH-containing lipoprotein associates (NCL), Stevia phytopreparation, Suprol.