학술논문
Absorption coefficient imaging by near-field scanning optical microscopy in bacteria
Document Type
Author Abstract
Source
Applied Optics. June 1, 2003, Vol. 42 Issue 16, p3005, 4 p.
Subject
Language
English
ISSN
1559-128X
Abstract
We present a method for obtaining a position-dependent absorption coefficient from near-field scanning optical transmission microscopy. We show that the optical transmission intensity can be combined with the topography, resulting into an absorption coefficient that simplifies the analysis of different materials within a sample. The method is tested with the dye rhodamine 6G, and we show some analysis in biological samples such as bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa. The calculated absorption coefficient images show important details of the bacteria, in particular for P. aeruginosa, in which membrane vesicles are clearly seen. OCIS codes: 170.5810, 170.1530, 180.5810, 300.1030.