부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biortech.2007.02.023 Byline: Bishnu P. Chapagain (a), Vinod Saharan (a)(b), Zeev Wiesman (a) Keywords: Balanites; Callus; Saponins; Larvicidal; Aedes Abstract: Seeking an alternative approach for producing a larvicidal product from Balanites aegyptiaca plants, callus was produced from in vitro cultures of root explants and its larvicidal activity against Aedes aegypti mosquito larvae was evaluated. Concentrations of 0, 50, 100, 500, 1000, and 1500ppm of saponins from the root-derived callus of B. aegyptiaca were used to determine larvicidal effects and consequent effect on adult emergence. A dose-dependent effect was observed. In a chronic mortality assessment (after 7 days of exposure), concentrations of 500ppm or greater killed 100% of the test larvae population. Fifty parts per million showed no difference in larval mortality compared to the control (0ppm); however, this concentration allowed one-fourth of the adult emergence of the control treatment. These results suggest that saponins from in vitro cultures of the root explant of B. aegyptiaca can be used as a larvicidal agent against A. aegypti larvae. Author Affiliation: (a) The Phyto-Lipid Biotechnology Laboratory, The Institutes for Applied Research, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel (b) Department of Biotechnology and Molecular Biology, Haryana Agricultural University, Hisar (Haryana) 125001, India Article History: Received 12 November 2006; Accepted 16 February 2007