학술논문
Defining Reference Ranges for Serum Anti-Mullerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility
Clinical Research Article
Clinical Research Article
Document Type
Academic Journal
Author
Benderradji, Hamza; Prasivoravong, Julie; Marcelli, Francois; Decanter, Christine; Rigot, Jean-Marc; Bongiovanni, Antonino; Sauve, Florent; Buee, Luc; Maurage, Claude-Alain; Cartigny, Maryse; Villers, Arnauld; Prevot, Vincent; Catteau-Jonard, Sophie; Sergeant, Nicolas; Giacobini, Paolo; Pigny, Pascal; Leroy, Clara
Source
Journal of Clinical Endocrinology & Metabolism. July 2022, Vol. 107 Issue 7, p1878, 10 p.
Subject
Language
English
ISSN
0021-972X
Abstract
Anti-Mullerian hormone (AMH) is a dimeric glycoprotein that belongs to the transforming growth factor-beta superfamily. It binds to its exclusive AMH type II receptor (AMH-R2), which has been shown to [...]
Background: Few studies to date have attempted to measure serum anti-Mullerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined in a large cohort. Objective: In this study, we aimed, first, to establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Second, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters. Methods: This single-center retrospective study included 578 normozoospermic adult men. Serum AMH concentrations were determined with an automated sandwich chemiluminescent immunoassay. Results: The median serum AMH was 43.5 pmol/L. The 2.5th and 97.5th percentile values for serum AMH and AMH/tT were 16.4 and 90.3 pmol/L and 0.45 and 3.43, respectively. AMH was positively correlated with inhibin B and sperm concentration and negatively correlated with age, follicle-stimulating hormone (FSH), and progressive sperm motility. Interestingly, using immunofluorescence, we documented for the first time that AMH type II receptor (AMH-R2) is expressed in ejaculated human spermatozoa and gonadotrophic cells in the postmortem pituitary gland. Conclusions: We establish a new age-specific reference range for serum AMH and AMH/tT. Moreover, AMH-R2 expression in human spermatozoa and gonadotrophic cells, together with the relationship between serum AMH levels and sperm motility or mean FSH levels, highlight new potential functions of AMH in regulating sperm motility or FSH secretion in adult men. Key Words: anti-Mullerian hormone, reference ranges, adult men, AMH-R2, FSH secretion, spermatozoa motility Abbreviations: AMH, anti-Mullerian hormone; AMH-R2, anti-Mullerian hormone type II receptor; AMH/tT, anti-Mullerian hormone-to-total testosterone ratio; AKAP4, A-kinase anchor protein 4; FSH, follicle-stimulating hormone; LH, luteinizing hormone; PBS, phosphate-buffered saline; T, testosterone.
Background: Few studies to date have attempted to measure serum anti-Mullerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined in a large cohort. Objective: In this study, we aimed, first, to establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Second, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters. Methods: This single-center retrospective study included 578 normozoospermic adult men. Serum AMH concentrations were determined with an automated sandwich chemiluminescent immunoassay. Results: The median serum AMH was 43.5 pmol/L. The 2.5th and 97.5th percentile values for serum AMH and AMH/tT were 16.4 and 90.3 pmol/L and 0.45 and 3.43, respectively. AMH was positively correlated with inhibin B and sperm concentration and negatively correlated with age, follicle-stimulating hormone (FSH), and progressive sperm motility. Interestingly, using immunofluorescence, we documented for the first time that AMH type II receptor (AMH-R2) is expressed in ejaculated human spermatozoa and gonadotrophic cells in the postmortem pituitary gland. Conclusions: We establish a new age-specific reference range for serum AMH and AMH/tT. Moreover, AMH-R2 expression in human spermatozoa and gonadotrophic cells, together with the relationship between serum AMH levels and sperm motility or mean FSH levels, highlight new potential functions of AMH in regulating sperm motility or FSH secretion in adult men. Key Words: anti-Mullerian hormone, reference ranges, adult men, AMH-R2, FSH secretion, spermatozoa motility Abbreviations: AMH, anti-Mullerian hormone; AMH-R2, anti-Mullerian hormone type II receptor; AMH/tT, anti-Mullerian hormone-to-total testosterone ratio; AKAP4, A-kinase anchor protein 4; FSH, follicle-stimulating hormone; LH, luteinizing hormone; PBS, phosphate-buffered saline; T, testosterone.