학술논문
Effect of early insulin therapy on nuclear factor IoB and cytokine gene expressions in the liver and skeletal muscle of high-fat diet, streptozotocin-treated diabetic rats
Document Type
Report
Author
Source
Acta Diabetologica. Sept, 2008, Vol. 45 Issue 3, p167, 12 p.
Subject
Language
English
ISSN
0940-5429
Abstract
To clarify the effect of early insulin therapy on nuclear factor IoB (NFIoB) pathway and inflammatory cytokine responses in the liver and skeletal muscle in type 2 diabetes. High-fat diet and low dose streptozotocin (STZ) induced diabetic rats were given NPH insulin or gliclazide for 3 weeks initiated at the 3rd day after STZ injection as early treatment and NPH for 3 weeks at 1 month as late treatment. Intraperitoneal glucose tolerance test (IPGTT) was performed at 3rd day after the end of treatment. Early interventions caused a decrease in glucose-insulin index in IPGTT, promoted glucose transporter 4 (Glut4) gene and protein expressions in muscle and reduced phosphoenolpyruvate carboxykinase (PEPCK) protein levels in the liver. There was an increase in inhibitor IoB (IIoB[alpha]) protein and a decrease in NFIoB p65 DNA binding activity. A decreased level in mRNAs encoding tumor necrosis factor (TNF)[alpha] in the liver and muscle and interleukin (IL)-1[beta] in the liver were observed. Our results suggested that early insulin treatment inhibits NFIoB activity and inflammatory cytokine responses in the liver and skeletal muscle that were involved in the amelioration of insulin resistance in type 2 diabetic rats.