학술논문
A Biphasic Pattern of Reproductive Hormones in Healthy Female Infants: The COPENHAGEN Minipuberty Study
Clinical Research Article
Clinical Research Article
Document Type
Clinical report
Author
Source
Journal of Clinical Endocrinology & Metabolism. September 2022, Vol. 107 Issue 9, p2598, 8 p.
Subject
Language
English
ISSN
0021-972X
Abstract
The early postnatal and transient activation of the hypothalamic-pituitary gonadal (HPG) hormone axis is termed minipuberty. It has been suggested to peak at around 2 to 3 months of age, [...]
Context: Minipuberty, a period of a transient activation of the hypothalamic-pituitary-gonadal (HPG) axis in both sexes, enables evaluation of gonadal function in infants suspected of hypogonadism. However, female minipuberty remains poorly elucidated. Objective: We aimed to establish continuous reference ranges for the most commonly used reproductive hormones and to evaluate the dynamics of the HPG axis in females aged 0 to 1 year. Design: The COPENHAGEN Minipuberty Study (ClinicalTrials.gov ID: NCT02784184), a longitudinal, prospective cohort study. Setting: Healthy infants from Copenhagen. Patients or Other Participants: A total of 98 healthy, term female infants followed with 6 examinations including venipuncture during the first year of life. Intervention(s): None. Main Outcome Measure(s): Serum concentrations of LH, FSH, inhibin B, anti-Mullerian hormone (AMH), estrone (E1), estradiol (E2), and SHBG were quantified using highly sensitive methods in 266 serum samples. Results: Reference ranges were established for LH, FSH, inhibin B, AMH, E1, E2, and SHBG. Two peaks were observed in normalized mean curves for all hormones. The first peaks were timed around postnatal days 15 to 27 followed by a general nadir for all hormones around days 58 to 92. The second peaks occurred around days 107 to 125 for inhibin B, AMH, E1, E2, and SHBG and days 164 to 165 for LH and FSH. Conclusions: We present age-related, continuous reference ranges of the most commonly used reproductive hormones and present novel data revealing a biphasic and prolonged female minipuberty. ClinicalTrials.gov ID: NCT02784184 Key words: minipuberty, female, hypothalamic-pituitary-gonadal axis, HPG axis, reproductive hormones, reference ranges Abbreviations: AMH, anti-Mullerian hormone; CV, coefficients of variation; E1, estrone; E2, estradiol; GAMLSS, Generalized Additive Model for Location, Scale and Shape; HPG, hypothalamic-pituitary-gonadal axis; LC-MS/MS, liquid chromatography tandem mass spectrometry; LOD, limits of detection; LOQ, limits of quantification
Context: Minipuberty, a period of a transient activation of the hypothalamic-pituitary-gonadal (HPG) axis in both sexes, enables evaluation of gonadal function in infants suspected of hypogonadism. However, female minipuberty remains poorly elucidated. Objective: We aimed to establish continuous reference ranges for the most commonly used reproductive hormones and to evaluate the dynamics of the HPG axis in females aged 0 to 1 year. Design: The COPENHAGEN Minipuberty Study (ClinicalTrials.gov ID: NCT02784184), a longitudinal, prospective cohort study. Setting: Healthy infants from Copenhagen. Patients or Other Participants: A total of 98 healthy, term female infants followed with 6 examinations including venipuncture during the first year of life. Intervention(s): None. Main Outcome Measure(s): Serum concentrations of LH, FSH, inhibin B, anti-Mullerian hormone (AMH), estrone (E1), estradiol (E2), and SHBG were quantified using highly sensitive methods in 266 serum samples. Results: Reference ranges were established for LH, FSH, inhibin B, AMH, E1, E2, and SHBG. Two peaks were observed in normalized mean curves for all hormones. The first peaks were timed around postnatal days 15 to 27 followed by a general nadir for all hormones around days 58 to 92. The second peaks occurred around days 107 to 125 for inhibin B, AMH, E1, E2, and SHBG and days 164 to 165 for LH and FSH. Conclusions: We present age-related, continuous reference ranges of the most commonly used reproductive hormones and present novel data revealing a biphasic and prolonged female minipuberty. ClinicalTrials.gov ID: NCT02784184 Key words: minipuberty, female, hypothalamic-pituitary-gonadal axis, HPG axis, reproductive hormones, reference ranges Abbreviations: AMH, anti-Mullerian hormone; CV, coefficients of variation; E1, estrone; E2, estradiol; GAMLSS, Generalized Additive Model for Location, Scale and Shape; HPG, hypothalamic-pituitary-gonadal axis; LC-MS/MS, liquid chromatography tandem mass spectrometry; LOD, limits of detection; LOQ, limits of quantification