학술논문
A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes
Document Type
Author abstract
Author
Goldberg, Susanne M.D.; Johnson, Justin; Busam, Dana; Feldblyum, Tamara; Ferriera, Steve; Friedman, Robert; Halpern, Aaron; Khouri, Hoda; Kravitz, Saul A.; Lauro, Federico M.; Li, Kelvin; Rogers, Yu-Hui; Strausberg, Robert; Sutton, Granger; Tallon, Luke; Thomas, Torsten; Venter, Eli; Frazier, Marvin; Venter, J. Craig
Source
Proceedings of the National Academy of Sciences of the United States. July 25, 2006, Vol. 103 Issue 30, p11240, 6 p.
Subject
Language
English
ISSN
0027-8424
Abstract
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.