부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.immuni.2005.08.007 Byline: Lijun Wen, Joni Brill-Dashoff, Susan A. Shinton, Masanao Asano, Richard R. Hardy, Kyoko Hayakawa Abstract: Antigen receptor-mediated signaling is critical for the development and survival of B cells. However, it has not been established whether B cell development requires a signal from self-ligand engagement at the immature stage, a process known as "positive selection." Here, using a monoclonal B cell receptor (BCR) mouse line, specific for the self-Thy-1/CD90 glycoprotein, we demonstrate that BCR crosslinking by low-dose self-antigen promotes survival of immature B cells in culture. In spleen, an increase in BCR signaling strength, induced by low-dose self-antigen, directed naive immature B cells to mature, not into the default follicular B cell fate, but instead into the marginal-zone B cell subset. These data indicate that positive selection can occur in developing B cells and that BCR signal strength is a key factor in deciding between two functionally distinct mature B cell compartments in the microenvironment of the spleen. Author Affiliation: Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, Pennsylvania 19111 Article History: Received 12 March 2005; Revised 12 August 2005; Accepted 17 August 2005 Article Note: (miscellaneous) Published: September 20, 2005