학술논문
Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola
Document Type
Academic Journal
Author
Source
Applied and Environmental Microbiology. Sept, 1993, Vol. 59 Issue 9, p3070, 6 p.
Subject
Language
ISSN
0099-2240
Abstract
Two genes of Erwinia herbicola were needed for the growth of Escherichia Coli on tyrosine as the only source of nitrogen. The tutA gene encoding tyrosine phenol lyase was cloned in E. coli. The enzyme was secreted at levels that were high in the E. coli. The secretions took place in the presence of isoprophyl-beta-D-thiogalactopyranoside or lactose which acted as an inducer. The synthesis of L-dihydroxyphenylalamine was accomplished with catechol, pyruvate and ammonia by induceed cells.