학술논문
CD45 negatively regulates tumour necrosis factor and interleukin-6 production in dendritic cells
Document Type
Author abstract
Source
Immunology. June, 2006, Vol. 118 Issue 2, p250, 7 p.
Subject
Language
English
ISSN
0019-2805
Abstract
To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-2567.2006.02363.x Byline: Jenny Piercy, Svetla Petrova, Elma Z. Tchilian, Peter C. L. Beverley Keywords: dendritic cells; Toll-like receptor; cytokines; cytokine receptors Abstract: Summary CD45 is known to regulate signalling through many different surface receptors in diverse haemopoietic cell types. Here we report for the first time that CD45.sup.-/- bone marrow dendritic cells (BMDC) are more activated than CD45.sup.+/+ cells and that tumour necrosis factor (TNF) and interleukin-6 (IL-6) production by BMDC and splenic dendritic cells (sDC), is increased following stimulation via Toll-like receptor (TLR)3 and TLR9. Nuclear factor-[kappa]B activation, an important downstream consequence of TLR3 and TLR9 signalling, is also increased in CD45.sup.-/- BMDC. BMDC of CD45.sup.-/- mice also produce more TNF and IL-6 following stimulation with the cytokines TNF and interferon-[alpha]. These results show that TLR signalling is increased in CD45.sup.-/- dendritic cells and imply that CD45 is a negative regulator of TLR and cytokine receptor signalling in dendritic cells. Article History: Received 23 November 2005; revised 2 February 2006; accepted 16 February 2006. Article note: Dr P. Beverley, The Edward Jenner Institute for Vaccine Research, Compton, Berkshire RG20 7NN, UK., Email: peter.beverley@jenner.ac.uk