학술논문
Functional expression and characterization of the wild-type mammalian renal cortex sodium/phosphate cotransporer and an 215R mutant in Saccharomyces cerevisiae
Document Type
Academic Journal
Author
Source
Biochemistry. Oct 12, 1999, Vol. 38 Issue 41, p13551, 9 p.
Subject
Language
ISSN
0006-2960
Abstract
Research was conducted to develop an expression system for the Na+/P(sub i) cotransporter in the Saccharomyces cerevisiae strain MB192. The yeast strain lacks the high-affinity phosphate transport system usually found in yeast cells and can only grow when phosphate is the growth medium at relatively high concentrations. Results indicate that the yeast expression system is a useful tool for the investigation of structure-function relationships of the renal sodium/phosphate cotransporter and that 215R is a part of the structure involved in phosphate recognition and considerably influences the rate of phosphate uptake by the NaPi-2 cotransporter.