부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1471-0528.2008.02039.x Byline: MH van der Gaast (a,b), NS Macklon (a,c), K Beier-Hellwig (d), CA Krusche (d), BCJM Fauser (a,c), HM Beier (d), I Classen-Linke (d) Keywords: Biopsy; endometrial; glycodelin A; leukaemia inhibitory factor; maturation; uterine secretion Abstract: Objective To compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies. Design Prospective study. Setting University Hospital. Population Healthy female volunteers attending a gynaecological outpatient clinic. Methods Endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound. Main outcome measures Progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined. Results All data were correlated to estradiol (E.sub.2) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r = 0.376, P =0.048) in endometrial fluid samples as well with serum levels of E.sub.2 (r = 0.568, P =0.001) and P (r = 0.408, P =0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples. Conclusions The measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation. Author Affiliation: (a )Department of Obstetrics and Gynaecology, Division of Reproductive Medicine, Erasmus Medical Centre, Rotterdam, the Netherlands (b )Department of Obstetrics and Gynaecology, Reinier de Graaf Hospital, Delft, the Netherlands (c )Department of Reproductive Medicine and Gynaecology, University Medical Centre, Utrecht, the Netherlands (d )Department of Anatomy and Reproductive Biology, RWTH Aachen University, Aachen, Germany Article History: Accepted 14 October 2008. Article note: Dr NS Macklon, Department of Reproductive Medicine and Gynaecology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, the Netherlands. Email n.s.macklon@umcutrecht.nl