부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2009.01.089 Byline: Suresh Anaganti (a), Johanna K. Hansen (a), Duc Ha (a), Yoonsoo Hahn (a), Oleg Chertov (b), Ira Pastan (a), Tapan K. Bera (a) Keywords: LRRC26; Prostate cancer; Breast cancer; Alternate transcript; Non-AUG initiation Abstract: CAPC (also known as LRRC26) is a new gene with restricted expression in normal tissues, and with expression in many cancers and cancer cell lines. We have identified and characterized a short-transcript of CAPC (S-CAPC). The nucleotide sequence analysis of CAPC mRNA showed that the transcription for S-CAPC starts at position +610 on the L-CAPC transcript. Interestingly, no translation initiation codon 'AUG' is present in this transcript. To determine if a non-AUG start site is utilized, the S-CAPC sequence was cloned into an expression vector with C-terminal myc and histidine tags, and transfected into 293T cells. Western blot and MALDI-TOF MS analysis on purified S-CAPC gave two distinct peaks at approximately 7.5kDa. N-terminal amino acid sequencing of the purified 7.5kDa protein product indicated that translation starts at the codon for cysteine on the S-CAPC transcript generating a 7.5kDa CAPC protein products translated from a non-AUG initiation site. Author Affiliation: (a) Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Room 5110, Bethesda, MD 20892-4264, USA (b) Protein Chemistry Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USA Article History: Received 13 January 2009