부산대학교 도서관

Only few studies have focus on animals that received Pilocarpine (Pilo) and did not develop behavioral status epilepticus (SE) and, whether they may become epileptic in the model's chronic phase. Previews works observed mossy fiber sprouting in the hippocampus of Non-SE (NSE) rats, while others observed spontaneous and recurrent seizures (SRS) 6-8 months after animals received Pilo. It is known that neuronal excitability is influenced by female hormones, as well as, the occurrence of SE in castrated and non-castrated female rats. However, it is not known whether females that received Pilo and did not show SE, may have SRS. The aim of this work was to investigate whether castrated and non-castrated female rats that did not show behavioral SE after Pilo, will develop SRS in the following one-year. For that, animals received 360 mg/kg of Pilo and were video monitored for 12 months. SE females from castrated and non-castrated groups became epileptic since the first month after drug injection. Epileptic behaviors were identified watching video monitoring recordings in the fast speed. Castrated and Noncastrated NSE animals showed behaviors resembling seizures described by Racine Scale stages 1-3. Motor alterations showed by NSE groups could be observed only when recordings were analyzed in slow speed. In addition, behavioral manifestations as, rhythmic head movements, sudden head movements, whole body movements and immobility were also observed in both, SE and NSE groups. We concluded that NSE female rats may have become epileptic. Adding to it, slow speed analysis of motor alterations was essential for the observation of NSE findings, which suggests that possibly many motor alterations have been underestimated in epilepsy experimental research. Keywords: Pilocarpine, experimental model, non-Status Epilepticus, female rats, video monitoring. Poucos sao os estudos com foco em animais que receberam Pilocarpina (Pilo) e nao desenvolveram status epilepticus (SE) comportamental e, se os mesmos se tornarao epilepticos na fase cronica do modelo. Autores observaram o brotamento das fibras musgosas no hipocampo de ratos Nao-SE (NSE), enquanto outros observaram crises espontaneas e recorrentes (CER) 6-8 meses apos receberam a droga. A excitabilidade neuronal e influenciada pelos hormonios femininos e, da mesma forma, a ocorrencia de SE em ratas castradas e nao-castradas. Entretanto, nao e sabido se as femeas que nao apresentam SE terao CER. O objetivo deste trabalho foi investigar se femeas castradas e nao castradas que nao tiveram SE comportamental apos a injegao de Pilo desenvolverao CER dentro de um ano. Para isto, os animais receberam 360 mg/kg de Pilo e foram videomonitorados por 12 meses. As femeas SE castradas e nao-castradas se tornaram epilepticas desde o primeiro mes pos Pilo. O comportamento epileptico foi identificado assistindo as gravagoes na velocidade rapida. As femeas NSE castradas e nao-castradas apresentaram comportamentos similares aos estagios 1-3 da Escala de Racine. As alteragoes motoras nestes grupos (NSE) foram observadas apenas quando as videomonitoragao foi analisada na velocidade lenta. Alem destas, manifestagoes comportamentais como movimentos ritmicos da cabega, movimentos subitos da cabega, movimentos de todo o corpo e imobilidade tambem foram observadas em ambos grupos, SE e NSE. Concluimos que as femeas NE podem ter se tornado epilepticas. Adicionado a isto, a analise das alteragoes motoras na velocidade lenta foi essencial para a observagao dos achados das femeas NSE, o que sugere que possivelmente muitas alteragoes motoras tem sido subestimados na pesquisa em epilepsia experimental. Palavras-chave: Pilocarpina, modelo experimental, sem-Status Epilepticus, ratas, videomonitoramento.
1. Introduction Pilocarpine (Pilo) experimental model of epilepsy was first described in male rats by Turski et al. (1983), and in female rats by Amado and Cavalheiro (1998). Behavioral, pathologic [...]