학술논문
Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase
Original research
Original research
Document Type
Report
Source
European Oral Research. April 2023, Vol. 57 Issue 1, p16, 6 p.
Subject
Language
English
ISSN
2651-2823
Abstract
Introduction Dental amalgam is a restorative material that has been used in dentistry for many years. The main ingredients of amalgam alloy are silver (Ag), tin (Sn), copper (Cu) and [...]
Purpose This study aims at determining the amount of mercury released over time from amalgam after treatment in healthy subjects and to examine the relation of mercury with serum MT-1 and SOD-1 levels. Materials and Methods Amalgam filling was applied to the 15 subjects aged 19-22 years and blood samples were collected before treatment and 1 day, 7 days, 21 days and 35 days after treatment. Mercury analysis of serum samples was performed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, MT-1 and SOD-1 levels in serum samples were measured using commercial enzyme-linked immunosorbent assay (ELISA). Friedman test and Spearman's correlation analysis was performed to analyse the data. p value was interpreted in significance level of 0.05. Results As a result of the analysis for MT-1, it was found that the values decreased over time and this decrease was statistically significant after 21 days (p Conclusion The results of the present study indicated that the amount of mercury released from the tested amalgam were found to be tolerable and no significant relationship was found between MT-1 and SOD-1. Keywords: Amalgam, antioxidants, mercury, metallothionein, superoxide dismutase
Purpose This study aims at determining the amount of mercury released over time from amalgam after treatment in healthy subjects and to examine the relation of mercury with serum MT-1 and SOD-1 levels. Materials and Methods Amalgam filling was applied to the 15 subjects aged 19-22 years and blood samples were collected before treatment and 1 day, 7 days, 21 days and 35 days after treatment. Mercury analysis of serum samples was performed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, MT-1 and SOD-1 levels in serum samples were measured using commercial enzyme-linked immunosorbent assay (ELISA). Friedman test and Spearman's correlation analysis was performed to analyse the data. p value was interpreted in significance level of 0.05. Results As a result of the analysis for MT-1, it was found that the values decreased over time and this decrease was statistically significant after 21 days (p Conclusion The results of the present study indicated that the amount of mercury released from the tested amalgam were found to be tolerable and no significant relationship was found between MT-1 and SOD-1. Keywords: Amalgam, antioxidants, mercury, metallothionein, superoxide dismutase