부산대학교 도서관

Introduction The ovarian hyperstimulation syndrome (OHSS), a typically fatal and iatrogenic side effect of fertility treatments, is connected to an acute, intense fluid migration from the vascular space to the [...]
Objective: We look at the immunoreactivity of cholinergic receptor muscarinic 1 (CHRM1) in the ovarian tissues of rats with ovarian hyperstimulation syndrome (OHSS) considering the possibility that the muscarinic activity may contribute to the pathophysiology of OHSS. Materials and Methods: In this study, 14 immature female Wistar Albino rats were divided into two groups at random. The rats were 22 days old. Rats in the control group (n=7) were 22 days old, while those in the OHSS group (n=7) received 10 IU follicle-stimulating hormone subcutaneously over the course of four days and 30 IU human chorionic gonadotropin (hCG) on the fifth day to induce ovarian hyperstimulation. All the rats were sacrificed after all the groups' ovaries and blood samples were collected at the conclusion of the experiment. The left ovarian tissues were kept in aluminum foil at -80 [degrees]C, while the right ovarian tissues were kept in 10% formalin. Tissue vascular endothelial growth factor (VEGF), interleukin (IL)-1[beta], IL-6, IL-10, tumor necrosis factor (TNF)-[alpha] and malondialdehyde (MDA) levels were measured by The Enzyme Linked Immunosorbent Assay technique in the ovarian tissues. CHRM1 immunoreactivity was scored immunohistochemically. Results: Ovarian weight, tissue IL-10, TNF-[alpha], VEGF and MDA levels, and CHRM1 immunoreactivity were significantly increased in the OHSS group. Conclusion: Increased levels of CHRM1 activity may play a role in the pathophysiology of OHSS. With further studies, the effect of luteinizing hormone and hCG on the ovarian and hypothalamic cholinergic system can be further investigated, and useful information can be obtained in determining OHSS prevention strategies. Keywords: Rat, OHSS, cytokine, CHRM1 Amac: Bu calismada ovaryan hiperstimulasyon sendromlu (OHSS) ratlarin ovaryan dokularinda kolinerjik reseptor muskarinik 1'in (CHRM1) immunreaktivitesine, muskarinik aktivitenin OHSS patofizyolojisine katkida bulunma olasiligi isiginda bakmayi amaclandi. Gerec ve Yontemler: Bu calismada 14 adet 22 gunluk immatur Wistar Albino disi rat rastgele iki gruba ayrildi. Grup 1'deki (n=7) ratlar (n=7) 22 gunluk iken, Grup 2'deki (n=7) ratlar dort gun boyunca subkutan 10 IU folikul stimulan hormone ve besinci gunde ovaryan hiperstimulasyonu induklemek icin 30 IU insan koryonik gonadotropin (hCG) aldi. Deney bitiminde tum gruplarin ovaryumlari ve kan ornekleri alindiktan sonra tum ratlar sakrifiye edildi. Sol over dokulari -80 [degrees]C'de aluminyum folyo icinde, sag over dokulari ise %10'luk formalinde saklandi. Ovaryan dokuda vaskuler endotelyal buyume faktoru (VEGF), interlokin (IL)-1[beta], IL-6, IL-10, tumor nekroz faktor (TNF)-[alpha] ve malondialdehid (MDA) seviyeleri Enzyme Linked Immunosorbent Assay teknigi ile olculdu. CHRM1 immunoreaktivitesi, immunohistokimyasal olarak skorlandi. Bulgular: Kontrol grubu ile karsilastirildiginda OHSS grubunda over agirligi, doku IL-10, TNF-a, VEGF ve MDA seviyeleri ve CHRM1 immunreaktivitesi acisindan istatistiksel olarak anlamli bir artis vardi. Sonuc: Artmis CHRM1 aktivitesi OHSS'nin patofizyolojisinde rol oynayabilir. Daha ileri calismalar ile luteinizan hormonu ve hCG'nin over ve hipotalamik kolinerjik sistem uzerindeki etkisi daha fazla arastirilabilir ve OHSS onleme stratejilerinin belirlenmesinde faydali bilgiler elde edilebilir. Anahtar Kelimeler: Rat, OHSS, sitokin, CHRM1