부산대학교 도서관

PreCRTM repair enzyme was applied to a variety of damaged forensic samples to obtain a successful STR result. A total of 122 samples were selected for the analysis. We analyzed STR allele recovery with repair reaction and Identifiler® plus amplification kit. Also, DNA quantification was performed with the Quantifiler® kit. As results, 89 samples repaired with the PreCRTM. Some lost alleles restored and STR peak heights increased upon repair with this kit. In contrast, non-specific peaks were observed in some other samples. For 18 samples among 42 samples, DNA concentration was increased after the recovery. In conclusion, PreCRTM repair enzyme will be efficiently applied to forensic samples which were damaged by various environments. However, the results were sporadic and sometimes deleterious. Therefore, the results of STR typing after the enzyme treatment must be carefully analyzed.