부산대학교 도서관

Glycans are now attracting much attention in the fields of biomarkers, stem cells, and biosimilar drugs. The combination of evanescent-field fluorescence scanner "GlycoStation™ Reader 1200" and lectin microarray "LecChip™" creates a highly sensitive platform for monitoring interactions between lectins and glycans among different technologies. Because of the high sensitivity and simple procedure, GlycoStation™ can be positioned as the most powerful differential analysis tool in screening novel biomarkers focusing on changes of glycan structures. Moreover, antibody-overlay, lectin microarray methodology applied to GlycoStation™, makes it more precise analysis tool. In this talk, the basic strategy of biomarker discovery using GlycoStation™ will be explained, introducing some examples of paraffin-embedded colon tissue arrays, and antibody-overlay lectin microarrays for detecting prostate cancer. In order to make the biomarker development process more efficient, an automated enrichment device of target biomarkers from serum (using antibodies and lectins) is under development, and will also be introduced. In another example, application of cell surface glycome profiling using GlycoStation™ will be described as a powerful method of characterizing stem cells. This work is a joint development project between three groups: GP Biosciences, National Institute of Advanced Industrial Science and Technology, and National Center for Child Health Development.