학술논문
The direct competitive ELISA based on monoclonal antibody for detecting okadaic acid in seafood
Document Type
Conference
Author
Source
Proceedings 2011 International Conference on Human Health and Biomedical Engineering Human Health and Biomedical Engineering (HHBE), 2011 International Conference on. :920-924 Aug, 2011
Subject
Language
Abstract
Okadaic acid (OA), a key diarrheic shellfish poisoning (DSP) toxin will possibly arouses DSP symptoms by consuming the contaminated shellfish. The DSP toxins are stable at high temperatures, and long-term DSP toxicity is carcinogenic. Therefore a fast and reliable analytical method for the detection of OA and analogues in shellfish is worth developing. In this paper, a direct competitive ELISA (dcELISA) for detecting OA in seafood was developed based on monoclonal antibody (McAb). The regression equation of direct competitive ELISA was y=−38.831X+130.25 with a coefficient correlation of R 2 =0.989 8. The linear range and the limit of detection (LOD) were 1.56–75 ng/mL and 1.09 ng/mL, respectively. The average recovery of OA-spiked sample was 86.05% with the coefficient of variation (CV) of 7.83%. The results indicated that the developed dcELISA is a fast, sensitive and convenient assay and could be used for detecting of OA in seafood.