학술논문
Simultaneous Holographic Microscopy and Raman Spectroscopy Monitoring of Human Spermatozoa Photodegradation
Document Type
Periodical
Author
Source
IEEE Journal of Selected Topics in Quantum Electronics IEEE J. Select. Topics Quantum Electron. Selected Topics in Quantum Electronics, IEEE Journal of. 22(3):27-34 Jun, 2016
Subject
Language
ISSN
1077-260X
1558-4542
1558-4542
Abstract
Coupling digital holography with high-specific Raman spectroscopy is an attractive means of identifying biochemical changes in cells by both physical topography and spectroscopy. We have used this approach to simultaneously study biochemical and morphological characteristics of human sperm cells irradiated with green laser radiation. Severe spermatozoa variations associated with a topological redistribution of the sample and a gradual decrease in the Raman signal intensity were detected in a label-free configuration. Importantly, at laser fluences where no morphological alterations were detected (30 MJ/cm 2 ), high specific spectral variations were monitored to evaluate the cell photodegradation.