학술논문

Measuring cellular structure at submicrometer scale with light scattering spectroscopy
Document Type
Periodical
Source
IEEE Journal of Selected Topics in Quantum Electronics IEEE J. Select. Topics Quantum Electron. Selected Topics in Quantum Electronics, IEEE Journal of. 7(6):887-893 Jan, 2001
Subject
Engineered Materials, Dielectrics and Plasmas
Photonics and Electrooptics
Light scattering
Spectroscopy
Particle scattering
Optical refraction
Optical scattering
Cancer
Solids
Biological tissues
Humans
Proteins
Language
ISSN
1077-260X
1558-4542
Abstract
We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells.