학술논문
Pulsatile microvascular blood flow imaging by short-time Fourier transform analysis of ultrafast laser holographic interferometry
Document Type
Conference
Author
Source
2015 8th Biomedical Engineering International Conference (BMEiCON) Biomedical Engineering International Conference (BMEiCON), 2015 8th. :1-5 Nov, 2015
Subject
Language
Abstract
We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an off-axis reference beam with a 50 kHz framerate camera. Videos of local Doppler contrasts were rendered numerically by Fresnel transformation and short-time Fourier transform analysis. This approach enabled instantaneous imaging of pulsatile blood flow contrasts in superficial blood vessels over 256 × 256 pixels with a spatial resolution of 10 microns and a temporal resolution of 20 ms.