학술논문
Spectrally and Repetition Rate Tunable Femtosecond Fiber Laser for Two-Photon Biomedical Imaging Applications
Document Type
Conference
Source
2023 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC), 2023 Conference on. :1-1 Jun, 2023
Subject
Language
ISSN
2833-1052
Abstract
The current extremely rapid development of biomedical imaging techniques, such as OCT and multiphoton microscopy, requires the development of light sources with very sophisticated parameters. We present a femtosecond laser emitting at the borderline of visible and near-infrared range (760–800 nm). The intended application is in two-photon excited fluorescence microscopy, in which a fluorophore simultaneously absorbs two photons from the excitation beam. Compared to traditional (single-photon) fluorescence microscopy, the two-photon variant allows deeper penetration into imaged tissue, lower phototoxicity, and guarantees confocality (without the need for an aperture) [1]. This technique makes it possible to image, for example, the retina of the eye [2]. The laser we propose is an all-fiber-based solution that is an alternative to the titanium-sapphire lasers currently in use, offering compactness so that it can be easily integrated into existing devices (such as microscopes or ophthalmoscopes).