부산대학교 도서관

Most Raman spectrometers require frequent calibration in order to provide accurate and repeatable results, and the calibration interval decreases for operation at high resolution. Wavenumber calibration commonly uses emission lines from gas-discharge lamps (e.g. mercury, neon, argon) that provide well-defined frequencies with very low uncertainties. However, it is often impractical to align lamps properly at the sample position, and the lamp emission lines provide absolute frequencies rather than Raman shifts. A more practical alternative is the use of a reference sample with a known Raman spectrum, which allows for the calibration of Raman spectrometer regardless of the excitation wavelength. Polystyrene is commonly used as a reference as it is easily available and practical to handle.