학술논문
Analysis of glycosides in traditional chinese medicine using electrochemical detection
Document Type
Conference
Author
Source
2010 3rd International Conference on Biomedical Engineering and Informatics Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on. 2:755-758 Oct, 2010
Subject
Language
ISSN
1948-2914
1948-2922
1948-2922
Abstract
High performance liquid chromatography (HPLC) method was coupled with pulsed amperometric detector, a kind of electrochemical detector by combining with post-column sodium hydroxide solution in order to analyze glycosides. Using this method, the contents of amygdalin, baicalin, naringin, and hesperidin in TCMs were determined with isocratic elution of 13% acetonitrile with 10 mM sodium phosphate buffer (pH 6.9). The coefficient of linear regression was 0.9997–0.9999 for concentrations between 1 and 100 µg/mL. The precision (RSD %) was less than 8.89% in Chong-Phyo-Tang and Run-Chang-Tang. This method showed high selectivity for analyzing glycosides without any interfering peaks. Therefore, glycosides in TCMs were conveniently analyzed without any pretreatment steps.