학술논문

Microfluidic tools for biological sample preparation
Document Type
Conference
Source
2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578) Microtechnologies in medicine and biology Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on. :556-559 2002
Subject
Components, Circuits, Devices and Systems
Bioengineering
Microfluidics
Acoustic signal detection
Laboratories
Pathogens
Protein engineering
Viruses (medical)
Microorganisms
Instruments
Purification
Filtration
Language
Abstract
Researchers at Lawrence Livermore National Laboratory are developing means to collect and identify fluid-based biological pathogens in the forms of proteins, viruses, and bacteria. To support detection instruments, we are developing a flexible fluidic sample preparation unit. The overall goal of this Microfluidic Module is to input a fluid sample, containing background particulates and potentially target compounds, and deliver a processed sample for detection. We are developing techniques for sample purification, mixing, and filtration that would be useful to many applications including immunologic and nucleic acid assays. Sample preparation functions are accomplished with acoustic radiation pressure, dielectrophoresis, and solid phase extraction. We are integrating these technologies into packaged systems with pumps and valves to control fluid flow and investigating small-scale detection methods.