학술논문
Design and Fabrication of a Novel “Turn-On” NIR Fluorescent Sensor Based on Triphenylamine-Isophorone for Cysteine Sensing and its Application to Drug Analysis and Bioimaging
Document Type
Periodical
Author
Source
IEEE Sensors Journal IEEE Sensors J. Sensors Journal, IEEE. 22(21):20200-20206 Nov, 2022
Subject
Language
ISSN
1530-437X
1558-1748
2379-9153
1558-1748
2379-9153
Abstract
Cysteine is a significant biothiol and plays a crucial role in physiological and clinical diagnosis. In the current research, we report a novel near-infrared (NIR) fluorescence sensor derived from triphenylamine-isophorone conjugate for the detection of cysteine. In the sensing pathway, an NIR fluorescence emitting moiety is released after a fast addition-cyclization of cysteine to the methacrylate group, which acts as an emission quencher. The detection platform demonstrates an excellent linear response to cysteine. It has been determined that the probe displays a fast response time (about 4 min), a low detection limit (12.4 nM), and a high signal-to-noise ratio. Easy and quick practical applications such as visual thin layer chromatography (TLC) plate and cotton swab have been established. Also, the current promising approach is effectively applied for the bio-imaging of cysteine in yeast cells and for drug analysis. Thus, this strategy allows the current probe to sense selectively cysteine over other biothiols in “turn-on” mode.