학술논문
Advanced EWOD-based digital microfluidic system for multiplexed analysis of biomolecular interactions
Document Type
Conference
Author
Source
2011 IEEE 24th International Conference on Micro Electro Mechanical Systems Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on. :153-156 Jan, 2011
Subject
Language
ISSN
1084-6999
Abstract
This paper presents a low-cost technique for the fabrication of complex electrowetting-on-dielectric (EWOD) digital microfluidic devices. Using this original technology, we have developed devices in which 560 electrodes are used to mix and split nl-size liquid droplets and transport them to 100 analysis spots patterned on a disposable plastic top plate. We demonstrate the multiplexing capability of the developed devices by creating on-chip arrays of droplets with various concentration gradients. Finally, automated biomolecular immobilization and hybridization assays are performed in nl-size droplets under numerous conditions simultaneously with only a limited number of stock solutions.