부산대학교 도서관

Quantifying expression levels of proteins with sub cellular resolution is critical to many applications ranging from biomarker discovery to treatment planning. In this paper, we present a fully automated method and a new metric that quantifies the expression of target proteins in immunohisto-chemically stained tissue microarray (TMA) samples. The proposed metric is superior to existing intensity or ratio-based methods. We compared performance with the majority decision of a group of 19 observers scoring estrogen receptor (ER) status, achieving a detection rate of 96% with 90% specificity. The presented methods will accelerate the processes of biomarker discovery and transitioning of biomarkers from research bench to clinical utility.