부산대학교 도서관

While the development of silicon-substrate microelectrode arrays has enabled chronic recording of single unit activity from multiple neurons simultaneously, accurate interpretation of the signals depend on the anatomical placement of the electrodes. Toward this end, this paper develops an in vivo method for identifying the placement of electrodes based on laminar analysis of ketamine-Xylazien-Induced field potential oscillations in rat motor cortex. The proposed method is based on finding the polarity reversal in laminar oscillations which is reported to appear in upper part of layer V in laminar High Voltage Spindles (HVSs) of rat cortical column. Analysis of histological images showed a 21 μm error in the estimate of the polarity reversal depth compared to the expected range (850-1050 μm). One out of the four rats did not undergo a phase reversal. Histology verified that the electrode was placed deeper than 1050 μm. We propose that this method can be used to determine an estimate of laminar electrodes implanted in rat motor cortex.