학술논문
Microfluidic Ping Pong for cytokine detection
Document Type
Conference
Author
Source
2011 IEEE 24th International Conference on Micro Electro Mechanical Systems Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on. :936-939 Jan, 2011
Subject
Language
ISSN
1084-6999
Abstract
Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 µm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.