학술논문

Insulin-secreting cells differentiation derived from human embryonic germ cells
Document Type
Conference
Source
2010 3rd International Conference on Biomedical Engineering and Informatics Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on. 4:1695-1699 Oct, 2010
Subject
Bioengineering
Communication, Networking and Broadcast Technologies
Computing and Processing
Signal Processing and Analysis
Robotics and Control Systems
Insulin
Stem cells
Sugar
Humans
Diabetes
In vitro
Immune system
embryonic germ cells(EGCs)
insulin-secreting cells
diabetes
human
Language
ISSN
1948-2914
1948-2922
Abstract
Diabetes mellitus affects millions of people in the world. Islet replacement strategies are becoming increasingly attractive options for patients at risk for severe diabetic complications. A major limitation of these approaches is the limited number of organs and the efficacy, safety and supply that restrict the available for islet transplantation. Recently, scientists have reported that stem cells promise a plentiful and flexible source for transplantation therapy. Here we used 10 ng/mL hepatocyte growth factor (HGF), 10 µM nicotinamide in PRMI 1640 to induce human embryonic germ cells (hEGCs) to differentiate into insulin producing cells. The results demonstrate that HGF and nicotinamide can promote the efficacy of insulin-secreting cells formation derived from embryoid bodies (EBs) derived hEGCs. The percentage of Dithizone (DTZ) positive, insulin, PDX-1, CK-19, Nestin positive in induced groups were significantly higher than the control. More importantly, levels of insulin-secreting after stimulation with high concentration glucose in inducing cells were significantly higher than the control (P