학술논문
Approaches for stoichiometry and distance determination of nanometer bio-complex by dual-channel single molecule imaging
Document Type
Conference
Author
Source
2009 IEEE/NIH Life Science Systems and Applications Workshop Life Science Systems and Applications Workshop, 2009. LiSSA 2009. IEEE/NIH. :124-127 Apr, 2009
Subject
Language
Abstract
A dual-channel imaging system with single fluorophore sensitivity was assembled in this lab. Inclusion of an integrated laser combiner was introduced to facilitate simultaneous dual-channel imaging. The imaging system has been applied to study the structure, stoichiometry, distance and function of the phi29 DNA packaging motor. Approaches including single molecule photobleaching, single molecule FRET and binomial distribution quantification were carried out to clarify the stoichiometry and distance of pRNA on the biologically active packaging motor. The results were statistically analyzed to access the copy number of pRNA, and the distance constraint was used to verify the 3D structure of the computer model of phi29 DNA packaging motor.