학술논문
Direct Laser Writing of Titanium Dioxide-Laden Retinal Cone Phantoms
Document Type
Conference
Author
Source
2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS) Micro Electro Mechanical Systems (MEMS), 2020 IEEE 33rd International Conference on. :1223-1226 Jan, 2020
Subject
Language
ISSN
2160-1968
Abstract
Adaptive optics-optical coherence tomography (AO-OCT) is a developing medical imaging modality that can spatially resolve individual photoreceptor cell and detect retinal pathologies in vivo. To date, however, a lack of standardized physical models - termed “phantoms” - that recapitulate the opto-structural properties of the retina impedes performance assessment, which could lead to inconsistencies in retinal pathology detection once AO-OCT reaches the clinic. Here we present a novel approach for manufacturing three-dimensional (3D) retinal cone outer segment (OS) phantoms via two-photon direct laser writing (DLW) of a photomaterial laden with titanium (IV) dioxide (TiO 2 ) nanoparticles. The ability to directly integrate light-scattering particles into biologically relevant architectures facilitates AO-OCT visibility of phantom components. Preliminary DLW results demonstrated TiO 2 nanoparticle-laden phantoms that effectively match cone dimensions and spacing at 1°, 2.5°, 5°, and 10° retinal eccentricities. Furthermore, preliminary AO-OCT experiments revealed that individual retinal cones could be optically resolved in phantoms with center-to-center spacing less than $4\ \mu \mathrm{m}$. These results suggest a promising pathway to produce biologically relevant retinal phantoms to standardize clinical AO-OCT imaging performance.