학술논문
Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast
Document Type
article
Author
Mariam Jaafar; Julia Contreras; Carine Dominique; Sara Martín-Villanueva; Régine Capeyrou; Patrice Vitali; Olga Rodríguez-Galán; Carmen Velasco; Odile Humbert; Nicholas J. Watkins; Eduardo Villalobo; Katherine E. Bohnsack; Markus T. Bohnsack; Yves Henry; Raghida Abou Merhi; Jesús de la Cruz; Anthony K. Henras
Source
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Subject
Language
English
ISSN
2041-1723
Abstract
The molecular events underlying the assembly and maturation of the early pre-60S particles during eukaryotic ribosome synthesis are not well understood. Here, the authors combine yeast genetics and biochemical experiments to characterise the functions of two important players of eukaryotic ribosome biogenesis, the box C/D snoRNP snR190 and the helicase Dbp7, which both interact. They show that the snR190 snoRNA acts as a RNA chaperone that assists the structuring of the 25S rRNA during the maturation of early pre-60S particles and that Dbp7 is important for facilitating remodeling events in the peptidyl transferase center region of the 25S rRNAs during the maturation of early pre-60S particles.