부산대학교 도서관

Origanum compactum Benth (O. compactum) is widely used traditionally in Morocco to treat a broad range of illnesses, including infectious diseases. The aim of this study was to evaluate the phytochemical composition, quality control, and antibacterial activity of O. compactum leaf and flower essential oil. First, a quality control study on soil and irrigation water was performed to determine whether there was any risk of heavy metals endangering human health or causing stress to the plants studied. Laboratory examination of the environmental quality of the researched species revealed an almost absolute absence of metals that could endanger human health or any abiotic stressor. The essential oil was extracted by hydrodistillation. Chemical characterization was performed using gas chromatography-mass spectrometry (GC/MS). The yield of essential oil (EO) obtained by hydrodistillation of O. compactum leaves and flowers and moisture content were 4.27% and 12.20%, respectively. GC/MS identified 35 volatile compounds in the studied EO majorly composed of thymol (38.59%) followed by carvacrol (26.65%), o-cymene (14.33), and γ-terpinene (11.22%). The antibacterial activity of O. compactum leaf and flower essential oil was evaluated using the solid-state diffusion method against five Gram-negative bacterial strains and a Gram-positive strain. The results show that the essential oil of O. compactum leaves and flowers has a considerable inhibitory effect against E. coli with an MIC = 0.35 µg/mL, E. pseudocoloides (MIC = 0.35 µg/mL), E. vekanda (MIC = 0.35 µg/mL), K. pneumoniae (MIC = 0.7 µg/mL), P. aeruginosa (MIC = 0.35 µg/mL), and S. aureus (MIC = 0.35 µg/mL).