학술논문
Robust in vitro assay for analyzing the neutralization activity of serum specimens against hepatitis B virus
Document Type
article
Author
Source
Emerging Microbes and Infections, Vol 8, Iss 1, Pp 724-733 (2019)
Subject
Language
English
ISSN
22221751
2222-1751
2222-1751
Abstract
ABSTRACTAnti-HBs is a well-known marker of protective capability against HBV. However, little is known about the association between the qAnti-HBs determined by immunoassays and the neutralization activity (NAT) derived from functional assays. We developed an in vitro assay for direct measurement of the NAT of human sera. The new assay was highly sensitive, with an analytical sensitivity of 9.6 ± 1.3 mIU/mL for the HBIG standard. For serum detection, the maximum fold dilution required to produce ≥50% inhibition (MDF50) of HBV infection was used as the quantitative index. In vitro NAT evaluations were conducted for a cohort of 164 HBV-free healthy individuals. The results demonstrated that the NAT positively correlated with the qAnti-HBs (R2 = 0.473, p