학술논문
Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
Document Type
article
Author
Source
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Subject
Language
English
ISSN
2041-1723
Abstract
The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.