학술논문
Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus
Document Type
article
Author
Source
Stem Cell Research, Vol 76, Iss , Pp 103332- (2024)
Subject
Language
English
ISSN
1873-5061
Abstract
We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1–60, NANOG and SSEA4) and in vitro differentiation into three germ layers.