학술논문
B and T Cell Phenotypic Profiles of African HIV-Infected and HIV-Exposed Uninfected Infants: Associations with Antibody Responses to the Pentavalent Rotavirus Vaccine
Document Type
article
Author
Adriana Weinberg; Jane Lindsey; Ronald Bosch; Deborah Persaud; Paul Sato; Anthony Ogwu; Aida Asmelash; Mutsa Bwakura-Dangarambezi; Benjamin H. Chi; Jennifer Canniff; Shahin Lockman; Simani Gaseitsiwe; Sikhulile Moyo; Christiana Elizabeth Smith; Natasha O. Moraka; Myron J. Levin; for the P1072 and Tshipidi Study Teams; Charles Fane; Dudu Kooreng; Tebogo J. Kakhu; Loeto Mazhani; Tumalano Sekoto; Lesedi Tirelo; Tshepo T. Frank; Mpho Raesi; Grace Kinabo; Boniface Njau; Anne Buchanan; Janeth Kimaro; Felistus Mbewe; Ellen Shingalili; Fyatilani Chirwa; Helen Bwalya Mulenga; Tapiwa Mbengeranwa; Taurai Beta; Ethel Dauya; Hilda Mujuru
Source
Frontiers in Immunology, Vol 8 (2018)
Subject
Language
English
ISSN
1664-3224
Abstract
We examined associations between B and T cell phenotypic profiles and antibody responses to the pentavalent rotavirus vaccine (RV5) in perinatally HIV-infected (PHIV) infants on antiretroviral therapy and in HIV-exposed uninfected (PHEU) infants enrolled in International Maternal Pediatric Adolescent AIDS Clinical Trials P1072 study (NCT00880698). Of 17 B and T cell subsets analyzed, PHIV and PHEU differed only in the number of CD4+ T cells and frequency of naive B cells, which were higher in PHEU than in PHIV. In contrast, the B and T cell phenotypic profiles of PHIV and PHEU markedly differed from those of geographically matched contemporary HIV-unexposed infants. The frequency of regulatory T and B cells (Treg, Breg) of PHIV and PHEU displayed two patterns of associations: FOXP3+ CD25+ Treg positively correlated with CD4+ T cell numbers; while TGFβ+ Treg and IL10+ Treg and Breg positively correlated with the frequencies of inflammatory and activated T cells. Moreover, the frequencies of activated and inflammatory T cells of PHIV and PHEU positively correlated with the frequency of immature B cells. Correlations were not affected by HIV status and persisted over time. PHIV and PHEU antibody responses to RV5 positively correlated with CD4+ T cell counts and negatively with the proportion of immature B cells, similarly to what has been previously described in chronic HIV infection. Unique to PHIV and PHEU, anti-RV5 antibodies positively correlated with CD4+/CD8+FOXP3+CD25+% and negatively with CD4+IL10+% Tregs. In conclusion, PHEU shared with PHIV abnormal B and T cell phenotypic profiles. PHIV and PHEU antibody responses to RV5 were modulated by typical HIV-associated immune response modifiers except for the association between CD4+/CD8+FOXP3+CD25+Treg and increased antibody production.