학술논문
Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma
Document Type
article
Author
Karolyny Martins Balbinot; Felippe José Almeida Loureiro; Giordanna Pereira Chemelo; Ricardo Alves Mesquita; Aline Maria Pereira Cruz Ramos; Rommel Thiago Jucá Ramos; Artur Luiz da Costa da Silva; Sílvio Augusto Fernandes de Menezes; Maria Sueli da Silva Kataoka; Sergio de Melo Alves Junior; João de Jesus Viana Pinheiro
Source
PeerJ, Vol 11, p e14349 (2023)
Subject
Language
English
ISSN
2167-8359
Abstract
Background Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. Methodology To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. Results Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). Conclusions The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.