학술논문
A technique for repeated blood and cerebrospinal fluid sampling from individual rats over time without the need for repeated anesthesia
Document Type
article
Author
Source
Scientific Reports, Vol 14, Iss 1, Pp 1-6 (2024)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract Ethical animal use follows the 3R’s: Replacement, Reduction and Refinement. Here, we present the use of simultaneous jugular vein and cisterna magna catheterization via a port system in rats for repeated fluid sampling for 14 consecutive days without loss of catheter patency. This technique allows repeated intra-animal sampling without anesthesia and, if used with pooling samples from a cohort of animals, replaces the need for terminal collections for sufficient sample volumes.