학술논문
Oral metformin transiently lowers post-prandial glucose response by reducing the apical expression of sodium-glucose co-transporter 1 in enterocytes
Document Type
article
Author
Lorea Zubiaga; Olivier Briand; Florent Auger; Veronique Touche; Thomas Hubert; Julien Thevenet; Camille Marciniak; Audrey Quenon; Caroline Bonner; Simon Peschard; Violeta Raverdy; Mehdi Daoudi; Julie Kerr-Conte; Gianni Pasquetti; Hermann Koepsell; Daniela Zdzieblo; Markus Mühlemann; Bernard Thorens; Nathalie D. Delzenne; Laure B. Bindels; Benoit Deprez; Marie C. Vantyghem; Blandine Laferrère; Bart Staels; Damien Huglo; Sophie Lestavel; François Pattou
Source
iScience, Vol 26, Iss 4, Pp 106057- (2023)
Subject
Language
English
ISSN
2589-0042
Abstract
Summary: Metformin (MET) is the most prescribed antidiabetic drug, but its mechanisms of action remain elusive. Recent data point to the gut as MET’s primary target. Here, we explored the effect of MET on the gut glucose transport machinery. Using human enterocytes (Caco-2/TC7 cells) in vitro, we showed that MET transiently reduced the apical density of sodium-glucose transporter 1 (SGLT1) and decreased the absorption of glucose, without changes in the mRNA levels of the transporter. Administered 1 h before a glucose challenge in rats (Wistar, GK), C57BL6 mice and mice pigs, oral MET reduced the post-prandial glucose response (PGR). This effect was abrogated in SGLT1-KO mice. MET also reduced the luminal clearance of 2-(18F)-fluoro-2-deoxy-D-glucose after oral administration in rats. In conclusion, oral metformin transiently lowers post-prandial glucose response by reducing the apical expression of SGLT1 in enterocytes, which may contribute to the clinical effects of the drug.