부산대학교 도서관

We hypothesised that hydrogenated fat (HF)-spray-coated β-carotene (βC) supplement could be used to increase plasma βC concentration and conception rates after embryo transfer (ET) in Hanwoo beef cows. In Experiment 1, 12 multiparous Hanwoo cows were fed one of four experimental diets in a triplicate 4 × 4 Latin square design for a 28-day period. Treatments included no βC addition (control), HF-uncoated βC (HFuβC), HF-spray-coated βC (HFβC), and HF-spray-coated βC and vitamin A (HFβCA). The cows under βC-supplemented treatments were fed 400 mg/day of βC, and a daily intake for vitamin A of HFβCA treatment was 30 000 IU/day as retinyl acetate. Blood was collected on days 0, 26, 27, and 28 to analyse βC and other metabolite concentrations. In Experiment 2, 199 Hanwoo cows with low fertility were randomly assigned to either control (n = 99) or HFβC treatments (n = 100) based on the results of Experiment 1. The oestrus of the cows was synchronised for ET. The HFβC group was fed from 4 weeks before to 4 weeks after ET with a daily intake of 400 mg βC. Pregnancy for conception rates was diagnosed on day 60 after ET, and blood was collected for βC concentrations on the day before ET. Supplementing βC resulted in a high plasma βC concentration (P