부산대학교 도서관

Intro: HIV-associated neurocognitive disorders (HAND) are a significant factor impacting the quality of life in HIV-1 patients. Kynurenic acid (KYNA), derived from tryptophan (Trp) in a physiological state, is a neuroprotective molecule associated with astrocytes. Previous results suggested KYNA level in the brain of HIV-1 gp120 transgenic mice (Tg mice) fed with Trp is higher than that in the control group, and Tg mice were successfully established for simulating the HAND. In this study, Morris Water Maze (MWM) to determine behavioral changes in mice, evaluating the effect of KYNA on cognitive impairment in Tg mice. Methods: The mice were randomly numbered and divided into four groups (n=10): WT, WT+0.1%Trp, Tg mice, and Tg mice+0.1%Trp, fed for one month by grouping. All groups of mice intake water and food freely during this period. The data of the incubation period, crossing times, and the quadrant residence time were recorded by MWM and analyzed with SPSS 22.0. Findings: The residence time of the Tg mice+Trp group in the hidden platform quadrant was increased (P0.05). And it extended the incubation period of WT group without a statistical difference (P>0.05). Ultimately, the platform crossing times of both gp120+Trp group and WT+Trp group without a statistical difference (P>0.05). Those results suggested that MWM assessing learning and cognitive ability is feasible. Conclusion: MWM could evaluate the protective effect of KYNA on cognitive impairment in HIV-1 gp120 transgenic mice.