학술논문
Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism
Document Type
article
Author
Source
Communications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
Subject
Language
English
ISSN
2399-3642
Abstract
Souabni et al. introduce a new analytical method for the study of ABC tripartite efflux pumps, by using a lipid scaffold that mimics the protein’s native environment and a spectroscopic assay, employing quantum dots (QD), that monitors ATP hydrolysis and substrate transport in real-time. When applied to MacAB-TolC tripartite pump complex, results suggest high coupling efficiency between transport and energy consumption in the system.