학술논문
The IgG glycome of SARS-CoV-2 infected individuals reflects disease course and severity
Document Type
article
Author
Sterre L. Siekman; Tamas Pongracz; Wenjun Wang; Jan Nouta; Peter G. Kremsner; Pedro Vieira da Silva-Neto; Meral Esen; Andrea Kreidenweiss; Jana Held; Átila Alexandre Trapé; Rolf Fendel; Isabel Kinney Ferreira de Miranda Santos; Manfred Wuhrer; ImmunoCovid Consortium; Alessandro P. de Amorim; Jamille G M. Argolo; Rita de C.C. Barbieri; Marcelo Dias-Baruffi; Victor A F. Bastos; Vânia L D. Bonat; Cristina Ribeiro de Barros Cardoso; Ingryd Carmona-Garcia; Jonatan C S de Carvalho; Leticia F. Constant; Augusto M. Degiovani; Cassia F.S.L. Dias; Lúcia H. Faccioli; Marley R. Feitosa; Omar Feres; Ana Paula Morais Fernandes; Talita M. Fernandes; Thais F C. Fraga-Silva; Carlos Fuzo; Isabelle C. Guarneri; Cristiane M. Milanezi; Caroline T. Garbato; Gilberto Gambero Gaspar; Ângelo A.F. Júnior; Sandra R. Maruyama; Debora C. Nepomuceno; Nicola T. Neto; Camilla Narjara Simão Oliveira; Fátima M. Ostini; Rogerio S. Parra; Malena M. Pérez; Vinı́cius E. Pimentel; Giovanna da S. Porcel; José J R da Rocha; Lilian C. Rodrigues; Elisa M S. Russo; Dayane P. da Silva; Rafael C. da Silva; Carlos Arterio Sorgi; Camila O S. Souza; Diana M. Toro; Angelina L. Viana; Fernando Crivelenti Vilar; Ana C. Xavier; Kamila Zaparoli
Source
Frontiers in Immunology, Vol 13 (2022)
Subject
Language
English
ISSN
1664-3224
Abstract
Immunoglobulin G (IgG) antibodies play an important role in the immune response against viruses such as SARS-CoV-2. As the effector functions of IgG are modulated by N-glycosylation of the Fc region, the structure and possible function of the IgG N-glycome has been under investigation in relation to divergent COVID-19 disease courses. Through LC-MS analysis we studied both total IgG1 and spike protein-specific IgG1 Fc glycosylation of 129 German and 163 Brazilian COVID-19 patients representing diverse patient populations. We found that hospitalized COVID-19 patients displayed decreased levels of total IgG1 bisection and galactosylation and lowered anti-S IgG1 fucosylation and bisection as compared to mild outpatients. Anti-S IgG1 glycosylation was dynamic over the disease course and both anti-S and total IgG1 glycosylation were correlated to inflammatory markers. Further research is needed to dissect the possible role of altered IgG glycosylation profiles in (dys)regulating the immune response in COVID-19.