학술논문
DNA Vaccine Administered by Cationic Lipoplexes or by In Vivo Electroporation Induces Comparable Antibody Responses against SARS-CoV-2 in Mice
Document Type
article
Author
Allegra Peletta; Eakachai Prompetchara; Kittipan Tharakhet; Papatsara Kaewpang; Supranee Buranapraditkun; Teerasit Techawiwattanaboon; Tayeb Jbilou; Pratomporn Krangvichian; Sunee Sirivichayakul; Suwimon Manopwisedjaroen; Arunee Thitithanyanont; Kanitha Patarakul; Kiat Ruxrungtham; Chutitorn Ketloy; Gerrit Borchard
Source
Vaccines, Vol 9, Iss 8, p 874 (2021)
Subject
Language
English
ISSN
2076-393X
Abstract
In view of addressing the global necessity of an effective vaccine in the SARS-CoV-2 pandemic, a plasmid DNA vaccine, expressing for the spike (S) protein and formulated in lipoplexes, was manufactured and tested for in vitro transfection and in vivo immunogenicity. Blank cationic liposomes of 130.9 ± 5.8 nm in size and with a zeta potential of +48 ± 12 mV were formulated using the thin-film layer rehydration method. Liposomes were complexed with pCMVkan-S at different N/P ratios. Ratios of 0.25:1 and 1:1 were selected according to their complex stability and controlled size compared to other ratios and tested in vitro for transfection studies and in vivo for immunogenicity. Both selected formulations showed enhanced neutralizing antibody responses compared to pCMVkan-S injected alone, as well as an increased T cell response. The titers observed were similar to those of intramuscular electroporation (IM-EP), which was set as an efficacy goal.