학술논문

Transcriptional profiling of MEF2-regulated genes in human neural progenitor cells derived from embryonic stem cells
Document Type
article
Source
Genomics Data, Vol 3, Iss C, Pp 24-27 (2015)
Subject
MEF2
Transcription factor
Microarray
Gene expression
Human neural stem cells
Neuronal differentiation
Genetics
QH426-470
Language
English
ISSN
2213-5960
Abstract
The myocyte enhancer factor 2 (MEF2) family of transcription factors is highly expressed in the brain and constitutes a key determinant of neuronal survival, differentiation, and synaptic plasticity. However, genome-wide transcriptional profiling of MEF2-regulated genes has not yet been fully elucidated, particularly at the neural stem cell stage. Here we report the results of microarray analysis comparing mRNAs isolated from human neural progenitor/stem cells (hNPCs) derived from embryonic stem cells expressing a control vector versus progenitors expressing a constitutively-active form of MEF2 (MEF2CA), which increases MEF2 activity. Microarray experiments were performed using the Illumina Human HT-12 V4.0 expression beadchip (GEO#: GSE57184). By comparing vector-control cells to MEF2CA cells, microarray analysis identified 1880 unique genes that were differentially expressed. Among these genes, 1121 genes were up-regulated and 759 genes were down-regulated. Our results provide a valuable resource for identifying transcriptional targets of MEF2 in hNPCs.